The method is based on a competitive colorimetric ELISA assay. The trenbolone antibody has been coated in the plate wells. During the analysis, sample is added along with the trenbolone-horseradish peroxidase (Trenbolone-HRP) conjugate. If the trenbolone residue is present in the sample, it will compete for the trenbolone antibody, thereby preventing the Trenbolone-HRP from binding to the antibody attached to the well. The resulting color intensity, after addition of the HRP substrate (TMB), has an inverse relationship with the trenbolone residue concentration in the sample.
Trenbolone is a xenobiotic anabolic compound with androgenic growth promoting activities which is prohibited for use in the EU member states. The Trenbolone kit has the following cross-reactivities for 17-ß-trenbolone (100%), 17a-trenbolone (63%), altrenogest (38%), 19-nortestosterone (2%). Endogenous androgens had < % cross-reactivities in this kit. The detection limit of trenbolone in urine samples is as low as ng/ml after a simple extraction step. Detailed information of this ELISA is given in the Product Information Sheet.